What is an Electron Microscpe?

A group of microscopes that uses a beam of accelerated electrons as a source of radiation is called electron microscopes.

What is Electron Microscopy?

Electron microscopy (EM) is a technique for obtaining high-resolution images of biological and non-biological samples. Electron microscopy is used in biomedical research to examine the exact structure of tissues, cells, organs, and macromolecular complexes. The high resolution of electron microscope images is due to the use of electrons as radiation sources.

Electron Microscope Vs. Optical (Light) Microscope

Because the wavelength of an electron beam is 100,000 times shorter than the wavelength of visible light photons, electron microscopes have far greater detection power than light microscopes. These microscopes can provide a structural image of the smallest objects with a resolution better than 50 picometers. Electron microscopes have a magnification of more than 10 million times, while optical microscopes have a resolution of 200 nanometers and a magnification of less than 2000 times due to the limitation of the diffraction phenomenon.

What are the Types of Electron Microscopes?

Types of electron microscopes include four main types: Transmission Electron Microscope (TEM) Scanning Electron Microscope (SEM) Field Emission Scanning Electron Microscopy (FESEM) Reflection electron microscope (REM) Scanning tunneling microscopy (STM)

Scanning electron microscopy is based on the emission of secondary electrons. Due to the fact that the secondary electrons have low energy, they have a small mean free path in solid materials and move at the surface to a depth of a few nanometers.

A transmission electron microscope is used to look at thin specimens (tissue sections, molecules, etc.) through which electrons pass and produce an image. TEM is in many ways similar to a conventional light microscope. TEM is used to image intracellular space (in thin sections), the structure of protein molecules (using the Metal Shadowing technique), the structure of molecules in viruses and skeletal filaments, and the arrangement of protein molecules in cell membranes.

What are the Advantages of Electron Microscopes?

Exceptionally high magnification Extremely high resolution Provides investigation in a greater depth of field

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elina

2 years ago

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